About the Book

This book provides comprehensive details on the advances in recovery, separation, and purification operations of bioprocess technology.

Written by over 30 international experts, Separation Processes in Biotechnology contains practical information on topics such as physical, chemical, and biochemical characterization of products and their contaminants ... recent advances in protein secretion systems, differential product release, and membrane permeabilization ... separation and concentration operations including ion-exchange, membrane processes, aqueous two-phase extractions, and precipitation ... purification operations including affinity chromatography, reversed-phase chromatography, hydrophobic interaction chromatography, ion exchange purification, and electrically driven separations ... computer methods in chemical engineering and recent developments in artificial intelligence, and automation in chromatography ... and more!

Encouraging communication between scientists and chemical engineers, Separation Processes in Biotechnology is a useful reference for biochemical, chemical, and process engineers; biotechnologists; protein, applied, and industrial chemists and biochemists; industrial microbiologists; and food technologists.

Contents

I. Separation Processes 1. Selection of Operations in Separation Processes 2. Chemical, Physical and Biochemical Concepts in Isolation and Purification of Proteins II. Product Release and Recovery 3. Protein Secretion Systems in Microbial and Mammalian Cells 4. Cell Disruption by Homogenizer 5. Bead Mill Disruption 6. Enzymatic Cell Lysis for Product Release 7. Chemical Permeabilization of Cells for Intracellular Product Release III. Concentration and Separation Operations 8. Membrane Systems 9. Ion-Exchange Processes 10. Aqueous Two-Phase Separations 11. Precipitation IV. Purification operations 12. Ion Exchange in Purification 13. Process Affinity Chromatography 14. Reversed-Phase and Hydrophobic 15. Analysis of Effluent Profiles in Large-Scale Liquid Chromatography 16. Electrically Driven Separation Processes: Analytical and Preparative Methods V. Design of Separation Processes 17. Synthesis of Downstream Processes 18. Automation in Protein Purification: The Use of Expert Systems for Control of Process Chromatography 19. Downstream processing Plant and Equipment 20. Downstream Process Economics.

About the Author