About the Book:

In "Pharmaceutical Microbiology: A Laboratory Manual," delve into the intricate world of pharmaceutical microbiology as you journey through nearly 50 hands-on experiments meticulously curated for B-Pharmacy students. Authored in alignment with the Pharmacy Council of India's syllabus, this manual is a comprehensive guide to both general and pharmaceutical microbiology, offering a blend of theoretical insights and practical applications. From understanding microorganisms crucial to pharmaceutical product quality control to executing experiments tailored for academic settings, this manual equips readers with the necessary knowledge and skills. With a focus on clarity and accessibility, complex concepts are demystified through lucid explanations and illustrative diagrams. Perfect for aspiring pharmacists seeking to master the nuances of experimental pharmaceutical microbiology, this manual is a must-have for students navigating the dynamic landscape of pharmaceutical science.

Contents:

Microbiology Laboratory: Basic Rules and Requirements

1.Pharmaceutical Microbiology and Basic Rules of Laboratory 2. Basic Requirements of Microbiology Laboratory 

Instrumentation

3. Microscope 4. Autoclave 5. Incubator 6. Hot Air Oven 7. Laminar Air Flow 8. pH Meter 9. Spectrophotometer 10. Colony Counter

Preparation of Culture Media, Sterilization and Culture Methods

11. Preparation and Sterilization of Media 12. Aseptic Technique and Transfer of Microorganisms 13. Growth Promotion Test of the Medium 14. Preparation of Agar Slants, Stabs and Plates 15. Preservation of Microbial Cultures

Techniques for Isolation of Pure Culture from Mixed Culture

16. Streak Plate Method 17. Pour Plate Method 18. Spread Plate Technique

Staining Techniques of Microorganisms

19. Preparation of a Bacterial Smear 20. Staining 21. Simple Staining 22. Negative Staining 23. Gram Staining 24. Acid-Fast Staining 25. Staining of Fungus by Lactophenol Cotton Blue

Biochemical Tests for Identification of Bacteria

26. IMViC Tests 27. Indole Test 28. Methyl Red and Voges-Proskauer Test (MR-VP Test) 29. Citrate Test 30. Carbohydrate Fermentation Test 31. Starch Hydrolysis Test 32. Gelatin Hydrolysis Test 33. H2S Production Test

Bacterial Motility

34. Wet Mount Method 35. Hanging Drop Method 36. Soft Agar and Agar Deep Tube Methods

Microbial Assay of Antibiotics

37. Microbial Assay of Antibiotics 38. Turbidimetric or Tube Assay Method

Sterility Testing of Pharmaceuticals

39. Sterility Testing of Pharmaceuticals

Environmental Monitoring

40. Environmental Monitoring by Settling Plate Method

Bacterial Analysis of Water

41. Bacterial Examination of Water by Multiple Tube Fermentation Test 42. Bacteriological Examination of Water by Membrane Filtration Method 43. Analysis of Water for Total Bacterial Population by Standard Plate Count

Microbial Taxonomy

44. Fungi 45. Bacteria

About the Authors:

Dr. G .Shyam Prasad is currently working as an Assistant professor of Microbiology, at Vaagdevi Degree and PG College, Naimnagar, Hanamkonda, Warangal. He has 17 years of Teaching Research and Industrial experience. He received his Ph.D. in 2007 from Department of Microbiology, Kakatiya University, Warangal and he is also awarded with CSIR-Research Associate ship, New Delhi, India. He is also recepient of UGC-DR.D.S.Kothari post doctoral fellowship, New Delhi, India (Department of Biochemistry, Osmania University, Hyderabad). He is actively engaged in teaching to B-Pharmacy, BSc and MSc Sudents. He also guided more than 12 graduation and post graduation projects. He is also serving as Editorial Advisory board member of “International Journal of Pharmacy and Biological sciences”. He is also Reviewer of more than 10 national and International journals.He has filed 2 patents and written 3 book chapters and published 26 research articles to his credit in various national and international Journals. His research interests are in the area of Biotransformation studies, Mycotoxins, Thermophilic fungi. He also collaborated with Sonia M.Tiquia-Arashiro, Professor of Microbiology, University of Michigan, US in writing a book.