1. A brief history of enzymology 2. Chemical bonds and reactions in biochemistry 3. Structural components of enzymes 4. Protein-ligand binding equilibria 5. Kinetics of single-substrate enzyme reactions 6. Chemical mechanisms in enzyme catalysis 7. Experimental measures of enzyme activity 8. Reversible inhibitors 9. Tight binding inhibitors 10. Time-dependent inhibition 11. Enzyme reactions with multiple substrates 12. Cooperativity in enzyme catalysis.